Biochem Biophys Res Commun 2000 May 19;271(3):610-4

Published erratum appears in Biochem Biophys Res Commun 2000 Jun 16;272(3):966

Poliovirus induces an early impairment of mitochondrial function by inhibiting
succinate dehydrogenase activity.

Koundouris A, Kass GE, Johnson CR, Boxall A, Sanders PG, Carter MJ

School of Biological Sciences, University of Surrey, Guildford, Surrey, United
Kingdom.

Poliovirus infection of COS-1 and T47D cells caused a rapid decrease in total
cell respiration, and this was attributed to an inhibition of mitochondrial
respiration. The stimulation of mitochondrial respiration by pyruvate plus
malate or succinate was impaired in saponin-permeabilised cells. However, this
inhibition could be overcome by the addition of N,N,N',N'-tetramethyl-1,
4-phenylenediamine and ascorbate. The activity of succinate dehydrogenase was
impaired in parallel with the inhibition of mitochondrial respiration during
poliovirus infection. This shows that mitochondrial function is profoundly
altered during poliovirus infection and that this occurs primarily through
inhibition of electron flow at complex II of the mitochondrial respiratory
chain. Copyright 2000 Academic Press.

PMID: 10814509, UI: 20275463